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Z702625
Sigma
Bio conjugation Protocols: Strategies and Methods
| ISBN-10: | 1-58829098-0 |
| ISBN-13: | 978-1-58829098-4 |
Description
| General description | Experts update the classic bioconjugation methods and introduce techniques that go beyond pure biomedical applications to include elements from organic synthesis, molecular biology, and materials science. These methods cover the preparation of protein conjugates using covalent and noncovalent conjugation, the synthesis of nucleic acid conjugates using a variety of labeling techniques, and approaches to semisynthetic conjugates of proteins. Additional chapters address on-chip synthesis to generate microarrays for the high-throughput analysis of RNA and DNA. |
Properties
| publication info | C. Niemeyer, Humana Press, 2004, 344 pp., hard cover |
Table Of Contents
| Table of Contents | PART I ANTIBODY AND ENZYME CONJUGATES 1. Streptavidin-Biotin Crosslinking of Therapeutic Enzymes With Carrier Antibodies: Nanoconjugates for Protection Against Endothelial Oxidative Stress 2. Characterization of Endothelial Internalization and Targeting of Antibody-Enzyme Conjugates in Cell Cultures and in Laboratory Animals 3. Smart Polymer-Streptavidin Conjugates 4. Conjugates of Peptides and Proteins to Polyethylene Glycols 5. Chemical Production of Bispecific Antibodies 6. Preparation of Immunoconjugates Using Antibody Oligosaccharide Moieties 7. Synthesis of Hapten-Protein Conjugates Using Microbial Transglutaminase PART II NUCLEIC ACID CONJUGATES 8. Fluorescent Sample Labeling for DNA Microarray Analyses 9. High-Density Labeling of DNA for Single Molecule Sequencing 10. Sequence-Specific DNA Labeling Using Methyltransferases 11. Hapten Labeling of Nucleic Acids for Immuno-Polymerase Chain Reaction Applications 12. Covalent Coupling of DNA Oligonucleotides and Streptavidin 13. Synthesis of Oligonucleotide-Peptide and Oligonucleotide-Protein Conjugates 14. Synthesis of Peptide Nucleic Acid-Peptide Conjugates PART III GLYCOSYL AND LIPID CONJUGATES 15. Protein Lipidation 16. Synthesis of Lipidated Peptides 17. In Vitro Semisynthesis and Applications of C-Terminally Modified Rab Proteins 18. Generation and Characterization of Ras Lipoproteins Based on Chemical Coupling 19. Conjugation of Glycopeptide Thioesters to Expressed Protein Fragments: Semisynthesis of Glycosylated Interleukin-2 20. Subtilisin-Catalyzed Glycopeptide Condensation PART IV BIOFUNCTIONALIZATION OF SURFACES 21. Peptide Nucleic Acid Microarrays 22. Synthesis and Characterization of Deoxyribonucleic Acid-Conjugated Gold Nanoparticles 23. Biofunctionalization of Carbon Nanotubes for Atomic Force Microscopy Imaging Index |
